A new world emerged in science with the discovery of the microscope, which enabled us to study tiny organisms whose existence was only hinted up to then. Since that time microbiology advanced to the science we know and routinely use today. Classical microbiology is still the base upon which most microbiologists work in the clinical setting, food industry and other facilities. However, with the advancement of genetics and molecular biology, new fields started emerging among others molecular diagnostics which is very popular today. The aim of our group is to connect the old microbiological postulates with the new development of genetics and so reach new frontiers in this field.
Since it is said that we are currently found in the „post-antibiotic era“ the aim of our research, first of all, is to detect new antimicrobial substances through disk diffusion and dilution methods. The flora of our country is very rich and there are many species that have an antibacterial effect on different bacterial strains. Another aspect of our research is the study of biofilms, which is the best example of the merger of classical microbiology and genetics. The approach of classical bacteriology is based on “the pure culture technique” which implies the culturing of bacteria, from various samples, on adequate enriched solid or liquid media. However, the growth of bacteria in pure cultures, on artificial media in vitro conditions, does not reflect their real growth in nature. In natural environments, like host tissues and on surfaces, most bacteria exist in biofilms.
Today many labs are facing the challenge of differentiating between pathogenic and nonpathogenic species. Through the advancement of sequencing techniques and bioinformatics, we are now able to detect minor differences in the genomes of these species based on genome size or specific genome regions. We intend to compare the genomes of bacterial and protozoan species that are biochemically and morphologically similar to develop new efficient and cost-effective methods for their differentiation.
The aim of our research group is to study a different aspect of microbial genetics by merging the classical microbiological approach with genetics and molecular biology.